. tropicalisParental meals concentration mg/mlFigure 1. Intergenerational adaptations to numerous stresses are evolutionarily conserved in multiple species of Caenorhabditis. (A) Phylogenetic tree of your Elegans group of Caenorhabditis species adapted from Stevens et al., 2020. Scale represents substitutions per site. (B) ERK5 Compound Percent of wild-type C. elegans (N2), C. kamaaina (QG122), C. briggsae (AF16), and C. tropicalis (JU1373) animals surviving after 24 hr on plates seeded with P. vranovensis BIGb0446. Data presented as imply values s.d. n = three experiments of 100 animals. (C) Percent of C. kamaaina wild-type (QG122) animals surviving Figure 1 continued on subsequent pageBurton et al. eLife 2021;10:e73425. DOI: doi.org/10.7554/eLife.25 12 .five 6. three 3. 1 1. 6 2 12 five .five 6. 3 three. 1 2 12 5 .five six. three 3. 1 1. 6 25 12 .5 six.four ofResearch article Figure 1 continuedEvolutionary Biology | Genetics and Genomicsafter 24 hr of exposure to P. vranovensis. Information presented as mean values s.d. n = three experiments of 100 animals. (D) Percent of wild-type animals mobile and establishing at 500 mM NaCl just after 24 hr. Information presented as mean values s.d. n = 3 experiments of 100 animals. (E) Percent of wild-type and Cbr-gpdh-2(syb2973) mutant C. briggsae (AF16) mobile and creating after 24 hr at 500 mM NaCl. Information presented as mean values s.d. n = three experiments of 100 animals. (F) % of animals exhibiting detectable infection by N. parisii as determined by DY96 staining following 72 hr for C. elegans and C. briggsae, or 96 hr for C. kamaaina and C. tropicalis. Data presented as mean values s.e.m. n = 3 experiments of 8302 animals. (G) Boxplots for length of L1 progeny from P0 parents that had been subject for the HB101 dose series. Larvae had been measured making use of Wormsizer. Boxplots show median length with four quartiles. n = three experiments of 5000 animals. p 0.01, p 0.0001, p 0.0001. The on the internet version of this article contains the following figure supplement(s) for figure 1: Supply data 1. Statistics source data for Figure 1. Figure supplement 1. Intergenerational responses to environmental strain are conserved in wild isolates of Caenorhabditis species.Parental exposure to environmental stresses leads to prevalent and stress-specific gene expression changes in CK2 supplier offspring across speciesOf the four intergenerational models investigated right here, parental exposure of C. elegans to osmotic tension and P. vranovensis infection have been previously reported to cause substantial adjustments in offspring gene expression, such as the elevated expression of genes that are necessary for the observed intergenerational adaptations (Burton et al., 2020; Burton et al., 2017). These effects of parental anxiety exposure on offspring gene expression resemble a subset of your transcriptional tension response observed in parental animals and could potentially prime offspring to respond for the similar strain (Burton et al., 2020). Here, we exposed C. elegans, C. briggsae, C. kamaaina, and C. tropicalis to either osmotic stress or P. vranovensis infection and subsequently performed RNA-seq on offspring to test: (1) when the precise heritable alterations in gene expression in response to each of these stresses are conserved across species and (2) if any adjustments in gene expression correlate together with the phenotypic differences in intergenerational responses to pressure we observed in the distinctive species. This evaluation allowed us to evaluate the effects of parental anxiety on offspring gene expression of 7587 single-copy orthologs which can be conse
