7.30; identified: C, 82.78, H, 7.31 . Methyl 2,3,4-tri-O-cinnamoyl-6-O-myristoyl–Dgalactopyranoside (eight). FTIR (KBr) (max): 1702 (-CO) cm-1. 1H-NMR (CDCl3, 400 MHz) ( ppm): H 7.75 7.52, 7.37 (three 1H, three d, J = 16.0 Hz, 3 PhCH = CHCO-), 7.54 (6H, m, Ar ), 7.28 (9H, m, Ar ), six.55, 6.16, six.07 (3 1H, three d, J = 16.1 Hz, 3 PhCH = CHCO-), 5.48 (1H, d, J = 8.two Hz, H-1), 5.34 (1H, dd, J = eight.two and ten.six Hz, H-2), five.05 (1H, dd, J = 3.2 and ten.six Hz, H-3), four.66 (1H, d, J = three.7 Hz, H-4), 4.40 (1H, dd, J = 11.2 and six.six Hz, H-6a), four.01 (1H, dd, J = 11.two and 6.8 Hz, H-6b), three.52 (1H, m, H-5), three.50 (3H, s, 1-OCH3), two.32 2H, m, CH 3(CH 2) 11CH 2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.25 20H, m, CH3(CH2)10CH2CH2CO-, 0.88 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 795.97. Anal Calcd. for C48H58O10: C, 72.52, H, 7.35; discovered: C, 72.53, H, 7.37 .Methyl 6-O-myristoyl-2,three,4-tri-O-(p-toluenesulfonyl)–Dgalactopyranoside (9). FTIR (KBr) (max): 1705 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz) ( ppm): H eight.03 (3 2H, m, Ar ), 7.94 (3 2H, m, Ar ), five.23 (1H, d, J = eight.two Hz, H-1), 5.08 (1H, dd, J = eight.0 and 10.5 Hz, H-2), four.77 (1H, dd, J = three.1 and 10.six Hz, H-3), four.53 (1H, d, J = 3.7 Hz, H-4), four.27 (1H, dd, J = 11.0 and six.five Hz, H-6a), four.11 (1H, dd, J = 11.1 and 6.8 Hz, H-6b), three.98 (1H, m, H-5), 3.46 (3H, s, 1-OCH3), 2.37 2H, m, CH3(CH2)11CH2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.27 20H, m, CH3(CH2)10CH2CH2CO-, 0.98 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 868.ten. Anal Calcd. for C42H58O13S3: C, 58.17, H, six.74; identified: C, 58.19, H, six.76 . Methyl 2,three,4-tri-O-(3-chlorobenzoyl)-6-O-myristoyl-D-galactopyranoside (ten). FTIR (KBr) (max): 1709 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz): H 8.05 (3H, m, Ar ), 7.96 (3H, m, Ar ), 7.55 (3H, m, Ar ), 7.38 (3H, m, Ar -H), five.63 (1H, d, J = 8.1 Hz, H-1), 5.21 (1H, dd, J = 8.2 and ten.six Hz, H-2), 5.01 (1H, dd, J = 3.1 and 10.six Hz, H-3), 4.65 (1H, d, J = three.7 Hz, H-4), 4.40 (1H, dd, J = 11.1 and six.six Hz, H-6a), 4.20 (1H, dd, J = 11.two and 6.eight Hz, H-6b), four.00 (1H, m, H-5), three.46 (3H, s, 1-OCH3), two.35 2H, m, CH3(CH2)11CH2CO-, 1.65 2H, m, CH3(CH2)10CH2CH2CO-, 1.24 20H, m, CH3(CH2)10CH2CH2CO-, 0.86 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 821.19. Anal Calcd. for C42H49O10Cl3: C, 61.50, H, 6.02; identified: C, 61.52, H, 6.03 .Antimicrobial screeningThe fifteen modified thymidine derivatives (20) have been subjected to antibacterial screening working with 5 bacterial strains: two Gram-positive strains, namely, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538, and 3 Gram-negative strains, namely, Escherichia coli ATCC 8739, Salmonella abony NCTC 6017 and Pseudomonas aeruginosa ATCC 9027. All the compounds have been dissolved in dimethylformamide (DMSO) to acquire a 2 Kinesin-14 site option (w/v). Also, antifungal activities of the compounds have been studied against two fungi strains, namely, Aspergillus niger ATCC 16,404 and Aspergillus flavus ATCC 204,304. These test micro-organisms (bacteria and fungi) have been obtained from the Department of Microbiology, University of Chittagong, Bangladesh. Disks soaked in DMSO have been made use of because the GSK-3α site unfavorable handle.Screening of antibacterial activityThe antibacterial spectra in the test derivatives had been obtained in vitro by the disk diffusion process [29]. This approach applied paper disks of four mm diameter plus a glass Petri-plate of 90 mmGlycoconjugate Journal (2022) 39:261diameter throughout the experiment. Sterile five (w/v) dimethyl sulfoxide (DMSO) option ready the synthesized compounds’ desired concentration and standard antibiotics. The pa
