together with the UDPGT domain (PF00201.18), 91 putative EuUGT genes have been identified and verified manually (Supplementary file S1 and S2), 81 of which had been previously annotated as UGTs [33]. Having said that, the other genes annotated as UDP-glycosyltransferase/glucosyltransferase, glycosyltransferase, or UGT were excluded as a result of absence of a PSPG box. All the putative EuUGTs contained a conserved PSPG motif at their C-terminal. The organization in the 91 EuUGT genes was also examined. It was shown that the majority of EuUGT genes (48, 52.7 ) had no intron. Among the EuUGT genes containing introns, 37 out of 43 (86.05 ) had only one intron. Four EuUGT genes contained two introns and 1 gene had 3 introns (Figure S1). The amino acid similarity in between person pairs of these sequences varied from 21 to one Topo II review hundred . Amongst them, 3 pairs of EuUGTs (GWHPAAAL014233 and GWHPAAAL012139; GWHPAAAL022859 and GWHPAAAL018716; GWHPAAAL025228 and GWHPAAAL008406) had one hundred amino acid sequence identity as well as the similar gene structure (Table S1), in spite of being localized in distinctive scaffolds. The other 29 EuUGTs were discovered to be situated in tandem VEGFR2/KDR/Flk-1 Compound inside the similar scaffold with a high similarity of up to 73 inside the amino acid sequence and practically identical gene structure (Table S1). The protein sequence lengths and molecular weights of the EuUGTs ranged from 261 (GWHPAAAL023819) to 569 (GWHPAAAL006284) amino acid residues, and 29.1 (GWHPAAAL23819) to 64.3 (GWHPAAAL006284) kDa, respectively. The estimations of theoretical pI ranged from 4.82 (GWHPAAAL014996) to 8.89 (GWHPAAAL005638). Out on the 91 EuUGTs, 17 had been predicted as stable proteins, and 74 proteins were predicted as unstable proteins. The grand typical hydropathicities of 91 EuUGTs have been calculated to recommend that most of the EuUGTs (83) are hydrophilic proteins. The subcellular areas on the 91 EuUGTs have been also predicated, with 63 within the cytoplasm, 14 inside the chloroplast, 11 around the plasma membrane, 1 in extracellular space, 1 inside the lysosome, and 1 inside the mitochondria. Detailed information is summarized in Table S2. two.2. Phylogenetic Evaluation of EuUGTs Originally, UGTs from the model plant A. thaliana were classified into 14 phylogenetic groups (A N) determined by the homology of their amino acid sequences [6]. Subsequently, four new groups (O, P, Q and R) were located in P. ginseng [13], O. sativa [9], and Malus domestica [34]. Based on this, 91 EuUGTs had been later clustered with the selected 246 UGTs (for additional detail, see the Supplies and Methods section) from other plants to construct a phylogenetic tree (Figure 1A). All of the UGTs pointed out above were divided into 16 groups (A-N, O and R), that are identical for the 18 identified groups, excluding groups P and Q. The UGTs of groups P and Q have been discovered in the other plants, such as Punica granatum L. [11], T. aestivum [8], and Camellia sinensis [15]. The EuUGTs have been categorized into 14 groups, like A to E, G to M, and O and R. In the set of EuUGTs analyzed, there had been no EuUGTs from groups F, N, Q, or P.Plants 2021, 10, x FOR PEER Assessment Plants 2021, ten,four 4of 22 ofFigure 1. Phylogenetic tree of UGTs in E. ulmoides, L. usitatissimum andand thaliana constructed by neighbor-joining Figure 1. Phylogenetic tree of UGTs in E. ulmoides, L. usitatissimum A. A. thaliana constructed by neighbormethod (A)system (A) plus the distribution of members in the phylogenetic groups amongst chosen plants species (B). joining along with the distribution of members inside the phylogenetic groups am
