Lson CJ, Emson Computer. Restoration of thalamostriatal projections in rat neostriatal
Lson CJ, Emson Pc. Restoration of thalamostriatal projections in rat neostriatal grafts: electron microscopic evaluation. J Comp Neurol. 1991; 303:224. [PubMed: 2005239] Yung KK, Bolam JP, Smith AD, Hersch SM, Ciliax BJ, Levey AI. Immunocytochemical localization of D1 and D2 dopamine receptors in the basal ganglia on the rat: light and electron microscopy. Neuroscience. 1995; 65:70930. [PubMed: 7609871]NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 1.Low energy (A,B) and CDK4 medchemexpress 40light microscopic views (C,D) of VGLUT1 (A,C) and VGLUT2 (B,D) immunolabeling of rat brain. Photos A and B HDAC6 list present views at a coronal level anterior to the anterior commissure; note the presence of VGLUT2 thalamostriatal terminals in cortical layer four in image B and also the conspicuous absence of VGLUT1 cortical terminals in cortical layer four in image A. The 40views reveal the presence of numerous varicosities in striatum immunolabeled for VGLUT1 (C) and VGLUT2 (D), with all the varicosities extra than twice as abundant for VGLUT1 as VGLUT2.J Comp Neurol. Author manuscript; accessible in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 2.CLSM images in (A,C,E) present a single field from a double-labeled section, displaying the full colocalization of immunolabeling in rat striatum for guinea pig (GP) antiVGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as shown by the full labeling overlap inside the merged image (E) for (A,C). Conversely, CLSM photos in (B,D,F) present a single field from a double-labeled section, showing the near absence of colocalization of immunolabeling in rat striatum for guinea pig (GP) anti-VGLUT1 (B) and rabbit (Rb) antiVGLUT2 (D), as shown by the meager overlap within the merged image (F) for (B,D).J Comp Neurol. Author manuscript; available in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure three.Detail of every CLSM image shown in Figure two. Photos in (A,C,E) present magnified views with the upper left from Fig. 2A,C,E, respectively. Similarly, pictures (B,D,F) present magnified views of the upper left from Fig. 2B,D,F, respectively. These magnified views make it far more feasible to resolve person terminals, and thereby confirm: 1) the comprehensive colocalization noticed in rat striatum for guinea pig (GP) anti-VGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as further evidenced by the comprehensive labeling overlap within the merged image (E) for (A,C); and two) the near absence of colocalization in rat striatum for guinea pigJ Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Lei et al.Pageanti-VGLUT1 (B) and rabbit anti-VGLUT2 (D), as shown by the absence of evident overlap inside the merged image (F) for (B,D).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 4.CLSM views of immunofluorescence for VGLUT1 (A) or VGLUT2 (B) in fields with fluorescent PHAL labeling of corticostriatal axons and terminals (C,D). Note that corticostriatal terminals in (C) immunolabel for VGLUT1 but these corticostriatal terminals in (D) do not immunolabel for VGLUT2. This can be observed a lot more clearly inside the mer.
