S supported by NIH grants DK065789 and AR055923 (FL). The confocal microscopy experiments have been supported in element by the NIH funded George O’Brien Center for Kidney Disease Investigation (P30DK079333), Kidney translational Investigation Core and also the Renal Division in the Washington University College of Medicine. We thank Masato Hoshi from the Sanjay Jain lab for technical assistance together with the confocal microscope.Dev Biol. Author manuscript; available in PMC 2016 April 01.Lim et al.PageReferenceAkiyama H, Chaboissier MC, Martin JF, MFAP4, Mouse (HEK293, His-Flag) Schedl A, de Crombrugghe B. The transcription issue Sox9 has vital roles in successive steps on the chondrocyte differentiation pathway and is necessary for expression of Sox5 and Sox6. Genes Dev. 2002; 16:2813?828. [PubMed: 12414734] Akiyama H, Kim J-E, Nakashima K, Balmes G, Iwai N, Deng JM, Zhang Z, Martin JF, Behringer RR, Nakamura T, de Crombrugghe B. Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors. Proceedings of the National Academy of Sciences with the United states of America. 2005; 102:14665?4670. [PubMed: 16203988] Bandyopadhyay A, Tsuji K, Cox K, Harfe BD, Rosen V, Tabin CJ. Genetic evaluation of your roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genet. 2006; 2:e216. [PubMed: 17194222] Barna M, Niswander L. Visualization of cartilage formation: insight into cellular properties of skeletal progenitors and chondrodysplasia syndromes. Dev Cell. 2007; 12:931?41. [PubMed: 17543865] Benazet JD, Pignatti E, Nugent A, Unal E, Laurent F, Cathepsin B, Human (HEK293, C-His) Zeller R. Smad4 is expected to induce digit ray primordia and to initiate the aggregation and differentiation of chondrogenic progenitors in mouse limb buds. Development. 2012; 139:4250?260. [PubMed: 23034633] Bi W, Deng JM, Zhang Z, Behringer RR, Crombrugghe B.d. Sox9 is expected for cartilage formation. Nature Genetics. 1999; 22:85?9. [PubMed: 10319868] DeLise AM, Fischer L, Tuan RS. Cellular interactions and signaling in cartilage development. Osteoarthritis Cartilage. 2000; 8:309?34. [PubMed: 10966838] Delise AM, Tuan RS. Evaluation of N-cadherin function in limb mesenchymal chondrogenesis in vitro. Developmental dynamics : an official publication of the American Association of Anatomists. 2002; 225:195?04. [PubMed: 12242719] Hall BK, Miyake T. All for a single and 1 for all: condensations and also the initiation of skeletal improvement. Bioessays. 2000; 22:138?47. [PubMed: 10655033] Javier AL, Doan LT, Luong M, Reyes de Mochel NS, Sun A, Monuki ES, Cho KW. Bmp indicator mice reveal dynamic regulation of transcriptional response. PloS one particular. 2012; 7:e42566. [PubMed: 22984405] Jiang T-X, Yi J-R, Ying S-Y, Chuong C-M. Activin Enhances Chondrogenesis of Limb Bud Cells: Stimulation of Precartilaginous Mesenchymal Condensations and Expression of NCAM. Developmental Biology. 1993; 155:545?57. [PubMed: 7679361] Joeng KS, Long F. The Gli2 transcriptional activator can be a essential effector for Ihh signaling in osteoblast improvement and cartilage vascularization. Improvement. 2009; 136:4177?185. [PubMed: 19906844] Jones MV, Calabresi PA. Agar-gelatin for embedding tissues before paraffin processing. BioTechniques. 2007; 42:569?70. [PubMed: 17515193] Kaartinen V, Nagy A. Removal in the floxed neo gene from a conditional knockout allele by the adenoviral Cre recombinase in vivo. Genesis. 2001; 31:126?29. [PubMed: 11747203] Kim Y, Murao H, Yamamoto K, Deng JM, Behringer RR, Nakamura T, Akiyama H. Generation of transgenic mice for conditional overexpression.