rabbit anti-GFP polyclonal antibody 9005

Additiona Information:
9 5 2 inhostrabbit|isotypeIgG|clonalitypolyclonal|concentration1 mg/mL|applicationsICC/IF, WB|available sizes100 g|rabbit anti-GFP polyclonal antibody 9005antibodyDatabase link:P42212Tested applicationsWB,ICC/IFRecommended dilutionsWB: 1:1000-5000 IF/ICC 1:2000-5000ImmunogenRecombinant AcGFP expressed in and purified from E. coliSize and concentration100g and 1 mg/mLFormliquidStorage Instructions2-8C for short term, for longer term at -20C. Avoid freeze / thaw cycles.Storage bufferPBS, 50% glycerol, 0.04% NaN3Purityaffinity purifiedClonalitymonoclonalIsotypeIgGCompatible secondariesgoat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720Isotype controlRabbit monoclonal – Isotype Controltarget relevanceProtein expression of GFP and GFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, GFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tagsand GFPProtein namesGreen fluorescent proteinGene namesGFP,GFPProtein familyGFP familyMass26886DaFunctionEnergy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.TissuesPhotocytes.StructureMonomer.Post-translational modificationContains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.BiotechnologyGreen fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Green fluorescent protein can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when Thr-203 is replaced by Tyr). Further generation of mutants led to more stable proteins (at 37 degrees Celsius for example) with brighter fluorescence and longer fluorescence lifetimes. Fluorescent proteins and their mutated allelic forms have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions (PubMed:17685514, PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105, PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy (PubMed:17685514).Target Relevance information above includes information from UniProt accession: P42212The UniProt Consortium|

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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