In G. sinensis below Salt Tension and also the Effect of Exogenou 0.05.Agriculture 2021, 11, x FOR PEER Overview Agriculture 2021, 11,11 of 21 10 ofFigure five. Effects of distinctive salt concentrations and addition of exogenous calcium ions on the Figure five. Effects+of various salt concentrations and addition of exogenous calcium ions around the contents of Na , K+ , and Ca2+ in G. sinensis seedlings. (A ) Na+ , K+ , and Ca2+ content in differcontents of Na+, K+, and Ca2+ in G. sinensis seedlings. (A ) Na+, K+, and Ca2+ content material in various ent tissue parts of G. sinensis at different salt concentrations and with distinct concentrations of tissue parts of G. sinensis at distinctive salt concentrations and with various concentrations of calcalcium CK is often a G. sinensis plant plant in standard hydroponic culture in Hogland Hogland nutrient cium ions.ions. CK is often a G. sinensisgrown grown in normal hydroponic culture in nutrient solusolution. S1 and G. sinensis plants treated with concentration of 100 of 100 mmol/L NaCl, retion. S1 and S2 areS2 are G. sinensis plants treated with concentrationand 200and 200 mmol/L NaCl, spectively; S1+C1, + C1, S1 + C2, andrepresent represent one hundred mmol/L the additionthe five, ten, and of 5, 10, respectively; S1 S1+C2, and S1+C3 S1 + C3 one hundred mmol/L NaCl with NaCl with of addition 15 mmol/LCaCl2, respectively. The ion content material ion content was measured and analyzed by inductively and 15 mmol/LCaCl2 , respectively. The was measured and analyzed by inductively coupled plasma optical emission spectrometry immediately after one week ofone week of plant remedy. Theaverage the coupled plasma optical emission spectrometry immediately after plant therapy. The information would be the data are SD of 3 independent Clevidipine-d7 manufacturer replicates. The mean values represented by the different letters were sigaverage SD of 3 independent replicates. The mean values represented by the different letters nificantly distinct in Tukey’s test at p 0.05. had been substantially distinct in Tukey’s test at p 0.05.three.10. Effects of of Exogenous Calcium on Plant Phenolic Compounds under Salt Pressure 3.10. Effects Exogenous Calcium on Plant Phenolic Compounds beneath Salt Tension Subsequently, we studied the accumulation of phenolic compounds in in distinct Subsequently, we studied the accumulation of phenolic compounds unique tissues tissues treated with one hundred mmol/L NaCl (S1), 100 NaCl + ten mmol/L CaCl (S1 + C2), and treated with 100 mmol/L NaCl (S1), 100 mmol/L mmol/L NaCl + ten mmol/L CaClnormal growth state (CK). Visualization of phenolic components was performed by hierarchical cluster evaluation (Figure 6). Twenty-three phenolic compounds and L-phenylalanine had been determined by the LC S technique in methanol extracts of roots, stems, and leavesAgriculture 2021, 11,11 ofof G. sinensis, and also the sorts of compounds measured for the differently treated samples showed no difference. It may be noticed in the heat map that these compounds have been mainly divided into 3 groups on the basis of their tissue-specific accumulation patterns and participated in salt strain responses to varying degrees. The C6C3-type compound (p-hydroxycinnamic acid, p-coumaric acid), the C6C3C6-type compound (naringenin, glycyrrhizin, genistein, quercetin), and L-phenylalanine have been mainly accumulated within the roots (Figure 7A). There was a slight enhance but not significant inside the case of salt treatment alone, as well as a really important raise in case of exogenous calcium addition. The C6C1type compound (two,5-dihydroxybenzoic acid) plus the.
