Ed in oMSCs treated with yEVs. With respect to pluripotency markers at RNA level, a statistically substantial lower of pluripotency markers (Nanog and Oct4) in yMSCs treated with oEVs plus a statistically important enhance in oMSCs treated with yEVs have been obtained. On the contrary, it was obtained with respect Vinculin expression. At proteomic level, a statistically considerable raise of 3 isoforms of Lamin A/C in yMSCs treated with oEVs and statistically significant decrease in oMSCs treated with yEVs had been obtained. Summary/conclusion: MSC-derived EVs and their self-renewal capacity have been influenced by age, as reveled by modifications in the expression of self-renewal markers like Nanog, Oct4, Vinculin and Lamin A/C. These findings are crucial for the understand the influence in the ageing on MSCs and to advance in the development EV-based therapies.PT06.Ex vivo expansion of SARS-CoV-2 E Proteins Formulation umbilical cord blood-derived haematopoietic stem cells working with extracellular vesicles of bone marrow stromal origin Corina Ghebes1; Jess Morhayim2; Marion Kleijer1; Bram van der Eerden3; Jeroen van de Peppel3; Eric Braakman2; Carlijn Voermans1 Division of Hematopoiesis, Sanquin Study, Amsterdam, The Netherlands; 2Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; 3Department of Internal Medicine, Erasmus University Health-related Center, Rotterdam, The NetherlandsPT06.Functionality of bone marrow mesenchymal stem cell-derived extracellular vesicles on self-renewal Juan Antonio Fafian Labora1; Miriam Morente-L ez2; Patricia D zBarreiro2; Pablo Fernandez-Pernas2; Mar Arufe Gonda1 Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade 1Grupo de Terapia Celular y Medicina Regenerativa (TCMRCHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade de Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain. Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain., A Coru , Spain; 2Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina, A Coru , SpainBackground: Mesenchymal stem cells (MSCs) are extremely relevant for regeneration of mesoderm tissues. The ADAMTS2 Proteins Recombinant Proteins promising function of MSCs in cellbased therapies and tissue engineering is restricted as a result of a decline pluripotency, proliferation and immunogenic potential with increasing donor age. Extracellular vesicles (EVs) have been recognizing as potent vehicles of intercellular communication on various biological systems. MSC-derived EVs present positive aspects more than cell-based therapy since it eliminates the security issues linked with injection of MSCs in patients and particularly useful for enhancing recovery from various illnesses. Right here, the influence of ageing on self-renewal functionality MSC-derived EVs working with an in vitro model was studied. Strategies: Two age groups from bone marrow MSCs of male Wistar rats were used, old (3 months old) and young (14 days old). EVs wereBackground: Ex vivo expansion of umbilical cord blood (UCB)-deri.
