Tients with diabetes. Methods: Individuals at Concord Hospital with suspected CAD gave written informed consent and were administered RIPC (sphygmomanometer around the arm, three 5 min cycles, n = 31) or sham (n = 29) prior to angiography, with recruitment ongoing. Blood was collected pre- and quickly post-RIPC/sham and plateletfree plasma generated. Global coagulation/fibrinolytic prospective was measured by overall haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and many fibrinolytic things by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Analysis institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have prospective as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying cause of heart attack and stroke, EV release may be dysregulated and their contents can mediate pro-inflammatory effects. Several markers happen to be previously identified on uEV like exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of those membrane bound carriers include things like microRNAs (miRs). miR-21 and miR-155 are essential regulatory miRs which are upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to have pro-atherogenic effects and miR-155 deficiency in murine models leads to reduced atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from individuals diagnosed with coronary artery disease (CAD), classified as symptomatic (non-ST-elevation G-CSF R/CD114 Proteins Storage & Stability myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (steady angina). uEVs from symptomatic and asymptomatic sufferers have been isolated through benchtop centrifugation. The concentration and size of uEVs were analysed by way of the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = 10 per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Results: uEV concentration in symptomatic sufferers (median; six.46E+9 particles/mL) was significantly decreased (p 0.05) in comparison with asymptomatic sufferers (median; 1.25E+10 particles/mL). CD11B+ uEVs were enhanced and CD16+ uEVs had been decreased CD136 Proteins Storage & Stability inside the symptomatic individuals (p 0.01). Additionally, the concentration of CD45+ EVs have been increased in symptomatic patients (p 0.001). While uEV miR-21 was unchanged, miR-155 expression was significantly enhanced in the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic possible. As CAD severity increases, uEV concentration is reduced, surface marker expression is altered and uEV miR-155 expression is improved. Funding: The Irish Investigation Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal illness Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Overall health Evaluative Sciences, Research Institute, The Hospital for Sick Young children,.
