The induction medium for osteogenesis was Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, Carlsbad, CA, Usa) supplemented with ten% FBS, 1027 M dexamethasone, .two mM ascorbic acid 2phosphate, and 10 mM glycerol two-phosphate (Sigma, St. Louis, Usa)
BM-MSCs (P4) ended up induced to differentiate into osteoblasts and adipocytes by using the...