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S supported by NIH grants DK065789 and AR055923 (FL). The confocal microscopy experiments had been supported in element by the NIH funded George O’Brien Center for Kidney Illness Research (P30DK079333), Kidney translational Research Core plus the Renal Division at the Washington University School of Medicine. We thank Masato Hoshi in the Sanjay Jain lab for technical assistance using the confocal microscope.Dev Biol. Author manuscript; accessible in PMC 2016 April 01.Lim et al.PageReferenceAkiyama H, Chaboissier MC, Martin JF, Schedl A, de Crombrugghe B. The transcription issue Sox9 has essential roles in successive methods on the LILRA2/CD85h/ILT1, Human (HEK293, His-Avi) chondrocyte differentiation pathway and is needed for expression of Sox5 and Sox6. Genes Dev. 2002; 16:2813?828. [PubMed: 12414734] Akiyama H, Kim J-E, Nakashima K, Balmes G, Iwai N, Deng JM, Zhang Z, Martin JF, Behringer RR, Nakamura T, de Crombrugghe B. Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors. Proceedings in the National Academy of Sciences with the United states of Serum Albumin/ALB, Human (Biotinylated, HEK293, His-Avi) America. 2005; 102:14665?4670. [PubMed: 16203988] Bandyopadhyay A, Tsuji K, Cox K, Harfe BD, Rosen V, Tabin CJ. Genetic analysis on the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genet. 2006; two:e216. [PubMed: 17194222] Barna M, Niswander L. Visualization of cartilage formation: insight into cellular properties of skeletal progenitors and chondrodysplasia syndromes. Dev Cell. 2007; 12:931?41. [PubMed: 17543865] Benazet JD, Pignatti E, Nugent A, Unal E, Laurent F, Zeller R. Smad4 is needed to induce digit ray primordia and to initiate the aggregation and differentiation of chondrogenic progenitors in mouse limb buds. Improvement. 2012; 139:4250?260. [PubMed: 23034633] Bi W, Deng JM, Zhang Z, Behringer RR, Crombrugghe B.d. Sox9 is expected for cartilage formation. Nature Genetics. 1999; 22:85?9. [PubMed: 10319868] DeLise AM, Fischer L, Tuan RS. Cellular interactions and signaling in cartilage improvement. Osteoarthritis Cartilage. 2000; eight:309?34. [PubMed: 10966838] Delise AM, Tuan RS. Evaluation of N-cadherin function in limb mesenchymal chondrogenesis in vitro. Developmental dynamics : an official publication of your American Association of Anatomists. 2002; 225:195?04. [PubMed: 12242719] Hall BK, Miyake T. All for 1 and one particular for all: condensations as well as the initiation of skeletal improvement. Bioessays. 2000; 22:138?47. [PubMed: 10655033] Javier AL, Doan LT, Luong M, Reyes de Mochel NS, Sun A, Monuki ES, Cho KW. Bmp indicator mice reveal dynamic regulation of transcriptional response. PloS 1. 2012; 7:e42566. [PubMed: 22984405] Jiang T-X, Yi J-R, Ying S-Y, Chuong C-M. Activin Enhances Chondrogenesis of Limb Bud Cells: Stimulation of Precartilaginous Mesenchymal Condensations and Expression of NCAM. Developmental Biology. 1993; 155:545?57. [PubMed: 7679361] Joeng KS, Extended F. The Gli2 transcriptional activator is really a vital effector for Ihh signaling in osteoblast development and cartilage vascularization. Improvement. 2009; 136:4177?185. [PubMed: 19906844] Jones MV, Calabresi PA. Agar-gelatin for embedding tissues prior to paraffin processing. BioTechniques. 2007; 42:569?70. [PubMed: 17515193] Kaartinen V, Nagy A. Removal with the floxed neo gene from a conditional knockout allele by the adenoviral Cre recombinase in vivo. Genesis. 2001; 31:126?29. [PubMed: 11747203] Kim Y, Murao H, Yamamoto K, Deng JM, Behringer RR, Nakamura T, Akiyama H. Generation of transgenic mice for conditional overexpression.

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