AKT1 [p Thr308] Antibody Summary
| Immunogen |
Synthetic peptide derived from a region of AKT that contains threonine 308.
|
| Modification |
p Thr308
|
| Localization |
Cytoplasmic and Nuclear
|
| Specificity |
AKT [Thr308]
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
AKT1
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
WB: Use at a concentration of 1:1000. Predicted molecular weight: 65 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
Reactivity Notes
Cross-reacts with Human and Mouse. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Dulbeccos PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for AKT1 [p Thr308] Antibody
- AKT
- Akt1
- EC 2.7.11
- EC 2.7.11.1
- PKB alpha
- PKBMGC99656
- PRKBA
- Protein kinase B
- Proto-oncogene c-Akt
- rac protein kinase alpha
- RAC-alpha serine/threonine-protein kinase
- RAC-alpha
- RAC-PK-alpha
- RACPKB-ALPHA
- v-akt murine thymoma viral oncogene homolog 1
Background
Phosphorylation site specific antibody selective for the phosphorylated form of the AKT protein containing a phosphate on threonine 308. The antibody has been shown to recognize AKT in NIH-3T3 cells stimulated with PDGF. AKT is a key effector of the PI3K pathway. Several studies have shown that a signalling pathway from PI3K to AKT may mediate some cellular responses of PI3K, including protection from apoptosis in PC12 cells. AKT accomplishes its cell survival-promoting function by inhibiting apoptosis by means of its ability to phosphorylate and inactivate BAD and caspase 9. The function of AKT is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (threonine 308) in the kinase core and hydrophobic phosphorylation site on the carboxyl terminus (serine 473). Threonine 308 is phosphorylated by the phosphoinositide-dependent kinase 1 (PDK 1).
