ATP Colorimetric/Fluorometric Assay Kit: Robust & Convenient Colorimetric & Fluorometric Assay to Measure ATP Levels within an Hour. 100 Assays.
Detection method- Fluorescent microscopy (Ex/Em 488/518 nm) to detect staines live cells; (Ex/Em 488/615) to detect stained dead cells
Applications: Stained live and dead cells can easily be visualized by fluorescence microscopy.
Kit Type
Fluorometric Assay Kit
Applications/Dilutions
Application Notes
ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence (Novus Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. Novus newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (WLmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 UM) of ATP in various samples. The kit provides sufficient reagents for 100 assays