BCL-W/BCL2L2 Antibody (16H12) Summary
| Immunogen |
This antibody is specific to Bcl-W
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| Specificity |
Purified rat monoclonal antibody IgG2a Kappa isotype raised against full length human bcl-w protein (AA 1-193). This antibody can be used for the specific detection of human and murine bcl-w by Western Blotting, immunoprecipitation and FACS analysis.
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
BCL2L2
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody is intended for the detection of human and murine bcl-w by Western Blot, immunoprecipitation and Flow Cytometry analysis. It should be noted that the levels of antibody quoted are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user. PROTOCOLS Western Blot In Western Blot analysis, it is recommended that the antibody be used at a concentration of 1-10mg/mL, which has been shown to detect endogenous bcl-w in 30mg of total protein from mouse brain extract, or 1×10^6Hela cells. Immunoprecipitation. For immunoprecipitation of endogenous levels of bcl-w from cell lines or tissue, it is recommended that the antibody be used at a concentration of 1-5mg/mL. It is necessary to use a minimum of 1×107 cells or 1mg of total protein from a tissue extract. Flow Cytometry. For Flow Cytometry analysis of bcl-w overexpressing cell lines, it is recommended that the antibody be used at a concentration of 1mg/mL. It is necessary to permeabilise the cell membrane prior to analysis.
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Reactivity Notes
Human, Mouse
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.4)
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| Preservative |
0.05% Bronidox
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| Purity |
Protein A or G purified
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Notes
Relevant Entrez Gene Numbers: NM_004050.3
Alternate Names for BCL-W/BCL2L2 Antibody (16H12)
- Apoptosis regulator Bcl-W
- BCL2L2
- Bcl2-L-2
- BCL2-like 2
- Bclw
- Bcl-w
- BCLWbcl-2-like protein 2
- KIAA0271BCL2-L-2
