Product: Ethacridine (lactate monohydrate)
CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6) Summary
| Immunogen |
Mouse myeloma cell line transfected with human CD40L (CD154)
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| Specificity |
TRAP1.3.6 recognises the human CD154 cell surface antigen, a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. TRAP1.3.6 binds to CD154 at an epitope distinct from the CD40 binding site. Immunogen: Mouse myeloma cell line transfected with human CD40L (CD154)
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD40LG
|
| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody can be used for flow cytometry.Flow Cytometry: Use 10ul of the suggested working dilution (1/10-1/50) to label 106 cells in 100ul.
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Reactivity Notes
Human,Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS pH 7.4
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for CD40 Ligand/TNFSF5 Antibody (TRAP1.3.6)
- CD154 antigen
- CD154
- CD40 antigen ligand
- CD40 Ligand
- CD40-L
- CD40LG
- CD40LIGM
- gp39
- hCD40L
- HIGM1
- T-B cell-activating molecule
- T-BAM
- T-cell antigen Gp39
- TNF-related activation protein
- TNFSF5
- TNFSF5IMD3
- TRAP
- TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
- tumor necrosis factor (ligand) superfamily member 5
- Tumor necrosis factor ligand superfamily member 5
Background
CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.
