Collagen I alpha 1 Overexpression Lysate (Native)

Collagen I alpha 1 Overexpression Lysate (Native) Summary

Immunogen	The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000079. The protein contains a C-terminal DDK tag.
Specificity	Homo sapiens Collagen I, mRNA.
Gene	COL1A1

Applications/Dilutions

Application Notes	This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW	136.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Collagen I alpha 1

Type	Overexpression
Protein State	Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.