Complement C5 Antibody (12F3) – BSA Free Summary
| Immunogen |
C5 isolated from human plasma adsorbed onto aluminum hydroxide gel
|
| Epitope |
Epitope specificity differs from that of Clone 03 but slightly overlap as determined by inhibition ELISA.
|
| Specificity |
Clone 12F3 is specific for human C5. No reaction is seen with plasma from patients deficient in C5.
|
| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Gene |
C5
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Clone 12F3 reacts strongly with C5. A strong reaction is seen with normal human plasma, and no reaction is seen with plasma from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western blotting after SDS-PAGE, Clone 12F3 reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only and no reaction is seen with plasma from C5 deficient patients.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
10mM Phosphate (pH 7.4) and 0.5M NaCl
|
| Preservative |
15mM Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for Complement C5 Antibody (12F3) – BSA Free
- anaphylatoxin C5a analog
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
- C5
- complement C5
- complement component 5
- CPAMD4MGC142298
- FLJ17816
- FLJ17822
Background
C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) The molecular mass of C5 is 190 kDa.
