Product: Zomepirac (sodium salt)
Cytokeratin 14 Antibody (DE-SPK14) Summary
| Immunogen |
The antibody reacts with the Keratin 14 antigen.
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| Specificity |
DE-SPK14 reacts specifically in immunoblotting with human keratin 14 (50 kD). In tissue sections this mAb provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingival, myoepithelial cells and squamous cell carcinomas.
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| Isotype |
IgG2b
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
KRT14
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| Purity |
Tissue culture supernatant
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Suitable for frozen sections / cell preparations. Also suitable for paraffin-embedded tissue when using the MICROWAVE method.Working dilution: 1:20, based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but optimal dilution should be tested by serial dilution. Dilute immediately before use with PBS or Tris. Positive control: Glandular
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Reactivity Notes
Human,Rat
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
No buffer
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| Preservative |
No Preservative
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| Purity |
Tissue culture supernatant
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Alternate Names for Cytokeratin 14 Antibody (DE-SPK14)
- CK14
- CK-14
- Cytokeratin 14
- cytokeratin-14
- EBS3
- EBS4
- K14
- keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
- keratin 14
- keratin, type I cytoskeletal 14
- keratin-14
- KRT14
- NFJ
Background
Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer. This antibody labels the basal layer of stratifying squamous and non-squamous epithelia. The staining pattern iscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas.
