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Product: Pimelic Diphenylamide 108 (analog)

DDX28 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 using the numbering given in entry NP_060850.1 (GeneID 55794).
Clonality
Polyclonal
Host
Rabbit
Gene
DDX28
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
DDX28 Lysate (NBL1-09791)

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX28 Antibody

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ11282
  • M
  • MDDX28probable ATP-dependent RNA helicase DDX28
  • Mitochondrial DEAD box protein 28
  • mitochondrial DEAD-box polypeptide 28

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.

PMID: 16818652

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Author: premierroofingandsidinginc