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Product: Pimethixene

FBXO4 Antibody Summary

Immunogen
FBXO4 (NP_036308.1, 1 a.a. ~ 387 a.a) full-length human protein.MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAV
Specificity
FBXO4 – F-box protein 4,
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FBXO4 Antibody

  • DKFZp547N213
  • F-box protein 4
  • F-box protein Fbx4
  • FBX4F-box only protein 4
  • FLJ10141

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

PMID: 8718419

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Author: premierroofingandsidinginc

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Product: Celecoxib

FBXO4 Antibody Summary

Immunogen
Peptide with sequence C-EHTSAVNKMFSRH corresponding to internal region according to NP_277019.1.
Epitope
C-EHTSAVNKMFSRH
Specificity
This antibody is expected to recognize isoform 1 and 2 (NP_036308.1; NP_277019.1).
Clonality
Polyclonal
Host
Goat
Gene
FBXO4
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 48 kDa band in human heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1 kDa band according to NP_036308.1. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-52158.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.

Alternate Names for FBXO4 Antibody

  • DKFZp547N213
  • F-box protein 4
  • F-box protein Fbx4
  • FBX4F-box only protein 4
  • FLJ10141

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

PMID: 25068893

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Author: premierroofingandsidinginc