Fibronectin/Anastellin Antibody (A17) – BSA Free Summary
| Immunogen |
Lysed bovine corneal endothelial cells and extracellular matrix.
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| Epitope |
Epitope is located in the 120kD cell binding fragment.
|
| Marker |
Mesenchymal Cells Marker
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| Specificity |
Clone A17 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). Clone A17 cross-reacts with human and chicken fibronectin. Other species have not been tested.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
FN1
|
| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Clone A17 can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with fibronectin directly coated onto the microtiter well. In Western blotting a dilution guideline of 1/100 has proved successful.
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Reactivity Notes
Bovine, Human, Chicken
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
10mM Phosphate (pH 7.4) and 0.5M NaCl
|
| Preservative |
15mM Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Fibronectin/Anastellin Antibody (A17) – BSA Free
- CIGDKFZp686O13149
- Cold-insoluble globulin
- DKFZp686F10164
- DKFZp686H0342
- DKFZp686I1370
- fibronectin 1
- fibronectin
- Fibronectin/Anastellin
- FNFINC
- FNZ
- GFND2ED-B
- LETSGFND
- migration-stimulating factor
- MSF
Background
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities
