GDF-8/Myostatin Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC210368 and based on accession number NM_005259. The protein contains a C-terminal DDK tag.
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| Specificity |
Homo sapiens myostatin (MSTN), mRNA.
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| Gene |
MSTN
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
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|
| Theoretical MW |
42.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
RIPA buffer
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Lysate Details for GDF-8/Myostatin
| Type |
Overexpression
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| Protein State |
Native
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Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for GDF-8/Myostatin Overexpression Lysate (Native)
- GDF8
- GDF-8
- GDF8growth differentiation factor 8
- growth/differentiation factor 8
- MSTN
- Myostatin
Background
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
