GTF2A2 Antibody Summary
| Immunogen |
The immunogen for this antibody was a sequence from 1a.a. to 109a.a. from TFIIA2(a 109a.a. long protein)
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| Localization |
Nucleus.
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| Specificity |
TFIIA2
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| Clonality |
Polyclonal
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| Host |
Chicken
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| Gene |
GTF2A2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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| Positive Control |
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Reactivity Notes
This antibody is reactive against Human, rat.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
No Preservative
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| Purity |
Immunogen affinity purified
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Alternate Names for GTF2A2 Antibody
- General transcription factor IIA subunit 2
- general transcription factor IIA, 2 (12kD subunit)
- general transcription factor IIA, 2, 12kDa
- HsT18745
- TF2A2
- TFIIA p12 subunit
- TFIIA
- TFIIA-12
- TFIIA-gamma
- TFIIAS
- Transcription initiation factor IIA gamma chain
- transcription initiation factor IIA subunit 2
Background
TFIIA (Transcription Factor IIA) is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. It interacts with TBP (the TATA binding protein). This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
