MUC-1 Antibody (M2F1) Summary
| Immunogen |
High molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule.
|
| Epitope |
This product binds within the GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.
|
| Localization |
Cell Membrane and Secreted
|
| Specificity |
This product efficiently recognizes underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. This product belongs to Mab clusters 6 and 7 according to ISOMB.No cross-reactivity with egg white avidin. It is not cross-reactive with normal serum proteins and human tissues of non-epithelial origin.
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| Isotype |
IgG
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
MUC1
|
| Purity |
Protein A purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Can be used for immunohistochemical staining of paraffin and frozen sections of breast, lung
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Reactivity Notes
Cross-reacts with Human.Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein A purified
|
Alternate Names for MUC-1 Antibody (M2F1)
- Breast carcinoma-associated antigen DF3
- Carcinoma-associated mucin
- CD227 antigen
- CD227
- DF3 antigen
- EMA
- Episialin
- H23 antigen
- H23AG
- KL-6
- MAM6
- MUC-1
- MUC1/ZD
- mucin 1, cell surface associated
- mucin 1, transmembrane
- Mucin-1
- Peanut-reactive urinary mucin
- PEM
- PEMMUC-1/SEC
- PEMT
- Polymorphic epithelial mucin
- PUMMUC-1/X
- tumor associated epithelial mucin
- Tumor-associated epithelial membrane antigen
- Tumor-associated mucin
Background
MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
