Product: 4-Acetamidobutanoic acid
PA28 Activator alpha Subunit/PSME1 Antibody Summary
| Immunogen |
Peptide with sequence C-KNFEKLKKPRGETK corresponding to C-Terminus according to NP_006254.1.
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| Epitope |
KNFEKLKKPRGETK
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| Specificity |
This antibody is expected to recognise isoform 1 (NP_006254.1).
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
PSME1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 30 kDa band observed in human lymph node, spleen, tonsil and peripheral blood mononuceocyte lysates (calculated MW of 28.7 kDa band according to NP_006254.1). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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| Theoretical MW |
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PA28 Activator alpha Subunit/PSME1 Antibody
- 11S regulator complex alpha subunit
- 11S Regulator Complex Subunit alpha
- 29-kD MCP activator subunit
- IFI5111
- IFI5111MGC8628
- IGUP I-5111
- Interferon gamma up-regulated I-5111 protein
- interferon-gamma IEF SSP 5111
- interferon-gamma-inducible protein 5111
- PA28 Activator alpha Subunit
- PA28A
- PA28alphaActivator of multicatalytic protease subunit 1
- proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
- Proteasome activator 28 subunit alpha
- proteasome activator complex subunit 1
- proteasome activator subunit-1
- PSME1
- REG alpha
- REGalpha
- REG-alpha
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.
