Product: Pilocarpine (nitrate)
PDE9A Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC216690 and based on accession number NM_002606. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 1, mRNA.
|
| Gene |
PDE9A
|
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
68.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
|
Lysate Details for PDE9A
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for PDE9A Overexpression Lysate (Native)
- CGMP-specific 3-5-cyclic phosphodiesterase type 9
- CGMP-specific 3-5-cyclic phosphodiesterase type 9, EC 3.1.4.1710phosphodiesterase PDE9A21
- EC 3.1.4.35
- FLJ90181
- high affinity cGMP-specific 3-5-cyclic phosphodiesterase 9A
- HSPDE9A2
- phosphodiesterase 9A
Background
The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
