PRMT5 Antibody Summary
| Immunogen |
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of Protein Arginine Methyltransferase 5 using the numbering given in entry NP_006100.2 (GeneID 10419).
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| Predicted Species |
Mouse (100%), Rat (100%), Porcine (100%), Primate (100%), Ferret (100%), Guinea Pig (100%), Monkey (100%), Canine (100%), Equine (100%), Rhesus Macaque (100%), Rabbit (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
PRMT5
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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| Theoretical MW |
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, White-tuftedear marmoset, Crab-eating macaque, European domestic ferret and Northern white-cheeked gibbon.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PRMT5 Antibody
- EC 2.1.1.-
- EC 2.1.1.125
- Histone-arginine N-methyltransferase PRMT5
- HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
- HRMT1L5
- IBP72
- Jak-binding protein 1
- JBP1
- protein arginine methyltransferase 5
- protein arginine N-methyltransferase 5
- Shk1 kinase-binding protein 1 homolog
- skb1 (S. pombe) homolog
- SKB1 homolog (S. pombe)
- SKB1
- SKB1HsSKB1 homolog
Background
Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.
