PUMA Antibody (10C5G1) Summary
| Immunogen |
Recombinant protein corresponding to amino acids 76 – 170 of human PUMA-alpha.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
BBC3
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Purity |
Immunogen affinity purified
|
Alternate Names for PUMA Antibody (10C5G1)
- BCL2 binding component 3
- bcl-2-binding component 3
- JFY1
- JFY-1
- p53 up-regulated modulator of apoptosis
- PUMAFLJ42994
Background
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
