Pappalysin-1/PAPP-A Antibody (10E1) Summary
| Immunogen |
Purified human PAPP-A antigen.
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| Specificity |
Human PAPP-A antigen
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| Isotype |
IgG2b
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
PAPPA
|
| Purity |
Protein A purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody works well in htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays, Immunoprecipitation and PAPP-A immunodetection in Western blotting. Recommended pair for the detection of dPAPP-A antigen in blood of AMI patients and patients with unstable angina (capture – detection): NB120-8346 (Capture) – NB100-73060 (Detection). Best pairs for the detection of htPAPP-A antigen in pregnancy serum (capture – detection): NB110-8423 (Capture) – NB120-8346 (Detection); NB120-8346 (Capture) – NB100-73060 (Detection). This antibody recognizes htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.
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Reactivity Notes
Cross-reacts with Human. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein A purified
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Alternate Names for Pappalysin-1/PAPP-A Antibody (10E1)
- ASBABP2
- DIPLA1
- EC 3.4.24.79
- IGFBP-4ase
- IGFBP-4aseaspecific BCL2 ARE-binding protein 2
- IGF-dependent IGFBP-4 protease
- insulin-like growth factor-dependent IGF binding protein-4 protease
- Insulin-like growth factor-dependent IGF-binding protein 4 protease
- PAPA
- PAPPA
- PAPPA1
- PAPP-A1
- PAPP-Adifferentially placenta 1 expressed protein
- Pappalysin1
- Pappalysin-1
- pregnacy-associated plasma protein A
- Pregnancy-associated plasma protein A
- pregnancy-associated plasma protein A, pappalysin 1
Background
PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).
