Product: Methicillin (sodium salt)
Phosphodiesterase 4D Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC212410 and based on accession number NM_006203. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (PDE4D), transcript variant 2, mRNA.
|
| Gene |
PDE4D
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Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
76.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
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Lysate Details for Phosphodiesterase 4D
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Phosphodiesterase 4D Overexpression Lysate (Native)
- cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3)
- cAMP-specific 3-5-cyclic phosphodiesterase 4D
- cAMP-specific phosphodiesterase PDE4D6
- DKFZp686M11213
- Drosophila)
- dunce-like phosphodiesterase E3
- EC 3.1.4
- FLJ97311
- HSPDE4D
- phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog
- phosphodiesterase 4D, cAMP-specific
Background
The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM]. Transcript Variant: This variant (2) differs in the 5 UTR and coding region, compared to variant 1. Isoform 2 is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
