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Product: TD139

RRAD Antibody Summary

Immunogen
Peptide with sequence C-HRRSMPVDERDLQ corresponding to internal region according to NP_004156.1.
Specificity
Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RRAD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 – 2 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 37 kDa band observed in mouse heart lysates and no 37 kDa band in the KO lysates (calculated MW of 33.2 kDa band according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than WB. In transfected HEK293 transiently expressing mouse Rrad (HA tagged), a band of approx. 45 kDa is observed. No bands are observed in transfected HEK293 transiently expressing related mouse GTPases.
Publications
Read Publication using NBP2-27500.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RRAD Antibody

  • RAD1
  • RADGTP-binding protein RAD
  • RAS (RAD and GEM) like GTP binding 3
  • Ras associated with diabetes
  • Ras-related associated with diabetes
  • REM3

PMID: 3720840

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Author: premierroofingandsidinginc

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Product: Tubeimoside I

RRAD Antibody Summary

Immunogen
Synthetic peptides corresponding to RRAD(Ras-related associated with diabetes) The peptide sequence was selected from the middle region of RRAD.Peptide sequence LARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASLMVYDIWEQDGGRWLPG.
Clonality
Polyclonal
Host
Rabbit
Gene
RRAD
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
Application Notes
This is a rabbit polyclonal antibody against RRAD and was validated on Western blot.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for RRAD Antibody

  • RAD1
  • RADGTP-binding protein RAD
  • RAS (RAD and GEM) like GTP binding 3
  • Ras associated with diabetes
  • Ras-related associated with diabetes
  • REM3

Background

RRAD may play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. RRAD regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane. RRAD inhibits cardiac hy

PMID: 23931998

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Author: premierroofingandsidinginc