Recombinant Human Apolipoprotein H/ApoH Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 20 – 345 of APOH.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSGRTCPKP DDLPFSTVVP LKTFYEPGEE ITYSCKPGYV SRGGMRKFIC PLTGLWPINT LKCTPRVCPF AGILENGAVR YTTFEYPNTI SFSCNTGFYL NGADSAKCTE EGKWSPELPV CAPIICPPPS IPTFATLRVY KPSAGNNSLY RDTAVFECLP QHAMFGNDTI TCTTHGNWTK LPECREVKCP FPSRPDNGFV NYPAKPTLYY KDKATFGCHD GYSLDGPEEI ECTKLGNWSA MPSCKASCKV PVKKATVVYQ GERVKIQEKF KNGMLHGDKV SFFCKNKEKK CSYTEDAQCI DGTIEVPKCF KEHSSLAFWK TDASDVKPC |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
APOH
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human Apolipoprotein H/ApoH Protein
- Activated protein C-binding protein
- Anticardiolipin cofactor
- APC inhibitor
- APOH
- apo-H
- apolipoprotein H (beta-2-glycoprotein I)
- Apolipoprotein H
- B2G1
- B2GP1
- B2GPI
- Beta
- beta(2)GPI
- beta-2-glycoprotein 1
- Beta-2-glycoprotein I
- BG
Background
Apolipoprotein H, also known as APOH, binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. This protein may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. Recombinant human APOH protein, fused to His-tag at N-terminus, was expressed in E.coli.
