Recombinant Human Biglycan Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 38 – 368 of BGN.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDEEASGADT SGVLDPDSVT PTYSAMCPFG CHCHLRVVQC SDLGLKSVPK EISPDTTLLD LQNNDISELR KDDFKGLQHL YALVLVNNKI SKIHEKAFSP LRKLQKLYIS KNHLVEIPPN LPSSLVELRI HDNRIRKVPK GVFSGLRNMN CIEMGGNPLE NSGFEPGAFD GLKLNYLRIS EAKLTGIPKD LPETLNELHL DHNKIQAIEL EDLLRYSKLY RLGLGHNQIR MIENGSLSFL PTLRELHLDN NKLARVPSGL PDLKLLQVVY LHSNNITKVG VNDFCPMGFG VKRAYYNGIS LFNNPVPYWE VQPATFRCVT DRLAIQFGNY KK |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
BGN
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
39.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.4M urea
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human Biglycan Protein
- BGN
- Biglycan
- Bone/cartilage proteoglycan I
- bone/cartilage proteoglycan-I
- dermatan sulphate proteoglycan I
- DSPG1
- PGI
- PG-S1
- SLRR1A
- SLRR1Abiglycan proteoglycan
- small leucine-rich protein 1A
Background
BGN, also known as biglycan Protein, is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. Recombinant human BGN protein, fused to His-tag at N-terminus, was expressed in E.coli.
