Recombinant Human CPOX Protein Summary
| Description |
A recombinant protein with a His tag corresponding to amino acids 111 – 454 of CPOX.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTSLGRPE EEEDELAHRC SSFMAPPVTD LGELRRRPGD MKTKMELLIL ETQAQVCQAL AQVDGGANFS VDRWERKEGG GGISCVLQDG CVFEKAGVSI SVVHGNLSEE AAKQMRSRGK VLKTKDGKLP FCAMGVSSVI HPKNPHAPTI HFNYRYFEVE EADGNKQWWF GGGCDLTPTY LNQEDAVHFH RTLKEACDQH GPDLYPKFKK WCDDYFFIAH RGERRGIGGI FFDDLDSPSK EEVFRFVQSC ARAVVPSYIP LVKKHCDDSF TPQEKLWQQL RRGRYVEFNL LYDRGTKFGL FTPGSRIESI LMSLPLTARW EYMHSPSENS KEAEILEVLR HPRDWVR |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
CPOX
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human CPOX Protein
- Coprogen oxidase
- coproporphyrinogen oxidase
- coproporphyrinogenase
- coproporphyrinogen-III oxidase, mitochondrial
- COX
- CPO
- CPXcoproporphyrinogen oxidase (coproporphyria, harderoporphyria)
- EC 1.3.3.3
- HCP
Background
Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria. Recombinant human CPOX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
