Product: CEP-28122 (mesylate salt)
Recombinant Human HB-EGF Protein Summary
| Description |
A recombinant protein with a His tag corresponding to amino acids 63 – 148 of HBEGF.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDLQEADL DLLRVTLSSK PQALATPNKE EHGKRKKKGK GLGKKRDPCL RKYKDFCIHG ECKYVKELRA PSCICHPGYH GERCHGLSL |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
HBEGF
|
| Purity |
>80% by SDS-PAGE and Coomassie blue staining
|
Applications/Dilutions
| Theoretical MW |
12.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>80% by SDS-PAGE and Coomassie blue staining
|
Alternate Names for Recombinant Human HB-EGF Protein
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
- Dtr
- DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
- Dts
- DTSF
- HBEGF
- HB-EGF
- Hegfl
- heparin-binding EGF-like growth factor
- heparin-binding epidermal growth factor
- proheparin-binding EGF-like growth factor
Background
HBEGF is growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function. This protein promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Recombinant human HBEGF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
