Recombinant Human Hexokinase 1 Protein | TLRsInhibitortlrsinhibitor

Recombinant Human Hexokinase 1 Protein Summary

Description	A recombinant protein with a His-tag corresponding to amino acids 1 to 917 of Human Hexokinase 1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS
Preparation Method	E.coli
Details of Functionality	Specific activity is > 3,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37C.
Protein/Peptide Type	Recombinant Protein
Gene	HK1
Purity	>90%, by SDS-PAGE

Applications/Dilutions

Application Notes

Assay
1. Prepare a 2.57ml reaction mixture into a suitable container: The final concentrations are 39 mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD.
2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 5ug of recombinant hexokinase1 into reaction mixture and mix immediately.
4. Record the increase at A340nm for 5 minutes

Theoretical MW

104.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Publications

Read Publication using NBC1-18390.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	20mM Tris-HCl pH8.0, 10% glycerol
Preservative	No Preservative
Concentration	1.0 mg/ml
Purity	>90%, by SDS-PAGE

Notes

The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 104.6kDa (937aa)

Alternate Names for Recombinant Human Hexokinase 1 Protein

Brain form hexokinase
EC 2.7.1
EC 2.7.1.1
glycolytic enzyme
Hexokinase 1
Hexokinase type I
hexokinase-1
HK I
HK1
HK1-ta
HK1-tb
HK1-tc
HKI
HXK1

Background

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

PMID: 15715459