Recombinant Human Hexokinase 2 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 917 of Human Hexokinase 2
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 12,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
HK2
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
Assay
1. Prepare a 2.57ml reaction mixture into a suitable container: The final concentrations are 39mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 5ug of recombinant hexokinase2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes. |
| Theoretical MW |
104.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl pH8.0, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Notes
Alternate Names for Recombinant Human Hexokinase 2 Protein
- DKFZp686M1669
- EC 2.7.1
- Hexokinase 2
- Hexokinase type II
- HK2
- HKII
- HXK2
- Muscle form hexokinase
- muscle
