Product: Quinagolide (hydrochloride)
Recombinant Human IL-1 beta/IL-1F2 Protein Summary
| Description |
An un-tagged recombinant protein corresponding to amino acids 117 to 269 of Human IL-1 beta/IL-1F2
Source: E.coli Amino Acid Sequence: MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS |
| Preparation Method |
E.coli
|
| Details of Functionality |
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or euqal to 0.005 ng/ml.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
IL1B
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
Use in In-vivo reported in scientific literature (PMID 26193793)
|
|
| Theoretical MW |
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffered saline (pH 7.4) pH7.4
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 17kDa (154aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein
- catabolin
- IL1 beta
- IL-1 beta
- IL-1
- IL1B
- IL-1b
- IL1-BETA
- IL-1F2
- IL1F2IL-1 beta
- interleukin 1, beta
- interleukin-1 beta
- preinterleukin 1 beta
- pro-interleukin-1-beta
Background
Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant human IL-1beta was expressed in E.coli and purified by using conventional chromatography techniques.
