Recombinant Human LC3B Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 120 of Human LC3B/MAP1LC3B
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG |
| Immunogen |
NCBI Accession No:NP_073729 Sequence of Amino Acid: MGSSHHHHHH SSGLVPRGSH LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR
|
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
MAP1LC3B
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 16.2 kDa (140aa), confirmed by MALDI-TOF
|
| Theoretical MW |
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl
|
| Concentration |
0.5 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human LC3B Protein
- Apg8b
- ATG8F
- Autophagy-related protein LC3 B
- Autophagy-related ubiquitin-like modifier LC3 B
- lc3b autophagy marker
- LC3B
- lc3-i, ii
- LC3II
- MAP1 light chain 3-like protein 2
- MAP1A/1BLC3
- MAP1A/MAP1B LC3 B
- MAP1LC3B
- microtubule associated protein 3 b
- microtubule associated protein 3 beta
- microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B
- microtubule-associated proteins 1A/1B light chain 3
- microtubule-associated proteins 1A/1B light chain 3B
Background
Microtubule-associated proteins 1A/1B light chain 3B, also known as MAP1LC3B, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. Recombinant human MAP1LC3B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
