Recombinant Human MPZL1 Protein Summary
| Description |
A recombinant protein with a His tag corresponding to amino acids 38 – 162 of MPZL1.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSLEVYTPK EIFVANGTQG KLTCKFKSTS TTGGLTSVSW SFQPEGADTT VSFFHYSQGQ VYLGNYPPFK DRISWAGDLD KKDASINIEN MQFIHNGTYI CDVKNPPDIV VQPGHIRLYV VEKENLPV |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
MPZL1
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
16.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human MPZL1 Protein
- FLJ21047
- immunoglobulin family transmembrane protein
- MPZL1b
- myelin protein zero-like 1
- myelin protein zero-like protein 1
- protein zero related
- Protein zero-related
- PZR1b
- PZRb
- PZRPZRa
Background
MPZL1 is a cell surface receptor, which is involved in signal transduction processes. This protein recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. It is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility. Recombinant human MPZL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
