Recombinant Human Melan-A/MART-1 Protein Summary
| Description |
A recombinant protein with a His tag corresponding to amino acids 48 – 118 of MLANA.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSCRRRNGY RALMDKSLHV GTQCALTRRC PQEGFDHRDS KVSLQEKNCE PVVPNAPPAY EKLSAEQSPP PYSP |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
MLANA
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
10.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human Melan-A/MART-1 Protein
- Antigen LB39-AA
- Antigen SK29-AA
- Mart 1 Melan A
- MART-1
- MART1MART-1
- MelanA
- Melan-A
- melanoma antigen recognized by T-cells 1
- MLANA
- Protein Melan-A
Background
Melanoma antigen recognized by T-cells 1, also known as MLANA, is involved in melanosome biogenesis by ensuring the stability of GPR143. This protein plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. Recombinant human MLANA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
