Recombinant Human PPP4C Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 1 – 307 of PPP4C.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PPP4C
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human PPP4C Protein
- EC 3.1.3.16
- Pp4
- PP4C
- PP4protein phosphatase X, catalytic subunit
- PPP4
- PP-X
- PPXPPH3
- protein phosphatase 4 (formerly X), catalytic subunit
- protein phosphatase 4, catalytic subunit
- Protein phosphatase X
- serine/threonine-protein phosphatase 4 catalytic subunit
Background
Serine/threonine-protein phosphatase 4 catalytic subunit, also known as PPP4C, belongs to the Serine/threonine-protein phosphatase catalytic subunits. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPP4C is a putative member of a novel PP family. The PPP4C may play a role in dephosphorylation and regulation of HDAC3. Recombinant human PPP4C protein, fused to His-tag at N-terminus, was expressed in E.coli.
