Recombinant Human Pentraxin 3/TSG-14 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 18 to 381 of Human Pentraxin 3/TSG-14
Source: E.coli Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS DDYDLMYVNL DNEIDNGLHP TEDPTPCDCG QEHSEWDKLF IMLENSQMRE RMLLQATDDV LRGELQRLRE ELGRLAESLA RPCAPGAPAE ARLTSALDEL LQATRDAGRR LARMEGAEAQ RPEEAGRALA AVLEELRQTR ADLHAVQGWA ARSWLPAGCE TAILFPMRSK KIFGSVHPVR PMRLESFSAC IWVKATDVLN KTILFSYGTK RNPYEIQLYL SYQSIVFVVG GEENKLVAEA MVSLGRWTHL CGTWNSEEGL TSLWVNGELA ATTVEMATGH IVPEGGILQI GQEKNGCCVG GGFDETLAFS GRLTGFNIWD SVLSNEEIRE TGGAESCHIR GNIVGWGVTE IQPHGGAQYV S |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PTX3
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 44.4 kDa (401aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Pentraxin 3/TSG-14 Protein
- alpha-induced protein 5
- pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor
- Pentaxin-related protein PTX3
- Pentraxin 3
- pentraxin 3, long
- pentraxin-3
- pentraxin-related gene, rapidly induced by IL-1 beta
- pentraxin-related protein PTX3
- PTX3
- TNF alpha-induced protein 5
- TNFAIP5
- TSG14
- TSG-14
- TSG-14pentaxin-related gene, rapidly induced by IL-1 beta
- Tumor necrosis factor alpha-induced protein 5
- tumor necrosis factor, alpha-induced protein 5
- Tumor necrosis factor-inducible gene 14 protein
- tumor necrosis factor-inducible protein TSG-14
Background
Pentraxin 3(PTX3) is the prototypic member of the long pentraxin family sharing the C-terminal domain with short pentraxins and containing a unique N-terminal domain. Pentraxin 3 is produced and released at inflammatory sites by diverse cell types including monocytes/macrophages, endothelial cells, vascular smooth muscle cells, fibroblasts, and adipocytes. It plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Recombinant human Pentraxin 3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
