Recombinant Human Prohibitin Protein Summary
| Description |
A recombinant protein with a His tag corresponding to amino acids 1 – 272 of PHB.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PHB
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human Prohibitin Protein
- PHB
- PHB1
- Prohibitin
Background
PHB is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3 untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3 untranslated region may function as a trans-acting regulatory RNA. Recombinant human PHB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
