Siglec-2/CD22 Antibody (MEM-01) [Biotin] Summary
| Immunogen |
Raji Burkitts lymphoma cell line
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| Epitope |
The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope “E”; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
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| Localization |
Type I membrane protein
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| Specificity |
The antibody MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD22
|
| Purity |
Protein A purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Western Blotting 120 min on vertical incubator Positive control: RAJI human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. The antibody MEM-01 stains only the zone corresponding to 140 kDa, but not the weaker 130 kDa zone (stained by several CD22 antibodies recognizing both isoforms). |
| Theoretical MW |
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Readout System |
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
15mM Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A purified
|
Alternate Names for Siglec-2/CD22 Antibody (MEM-01) [Biotin]
- B-cell receptor CD22
- BL-CAM
- B-lymphocyte cell adhesion molecule
- CD22 antigenMGC130020
- CD22 molecule
- CD22
- sialic acid binding Ig-like lectin 2
- Sialic acid-binding Ig-like lectin 2
- Siglec2
- Siglec-2
- SIGLEC2FLJ22814
- T-cell surface antigen Leu-14
Background
The antibody MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope “E”; it does not cross-block NB100-78574.
