TIP60 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC210591 and based on accession number NM_182709. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens K(lysine) acetyltransferase 5 (KAT5), transcript variant 3, mRNA.
|
| Gene |
KAT5
|
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
|
Lysate Details for TIP60
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for TIP60 Overexpression Lysate (Native)
- cPLA2
- ESA1EC 2.3.1.48
- Histone acetyltransferase HTATIP
- histone acetyltransferase KAT5
- HIV-1 Tat interactive protein
- HIV-1 Tat interactive protein, 60kDa
- HTATIP1
- HTATIPcPLA2 interacting protein
- K(lysine) acetyltransferase 5,60 kDa Tat-interactive protein
- K-acetyltransferase 5
- Lysine acetyltransferase 5
- PLIPTIP
- Tat interacting protein, 60kDa
- Tip60
- TIP60cPLA(2)-interacting protein
Background
The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (3), also known as beta, lacks two alternate in-frame segments, compared to variant 1, resulting in a shorter protein (isoform 3), that has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
