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Product: Sulfatinib

TNIK Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TNIK. The exact sequence is proprietary.
Predicted Species
Human (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNIK
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TNIK Antibody

  • EC 2.7.11
  • KIAA0551EC 2.7.11.1
  • TRAF2 and NCK interacting kinase
  • TRAF2 and NCK-interacting protein kinase

Background

Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM]

PMID: 19906957

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Author: premierroofingandsidinginc

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Product: PYZD-4409

TNIK Antibody Summary

Immunogen
Synthetic 19 amino acid peptide from internal region of human TNIK.
Specificity
Human TNIK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Mouse (95%), Rat (95%), Bat (95%), Bovine (95%), Chicken (95%), Canine (95%), Equine (95%), Opossum (95%), Xenopus (95%), Rabbit (95%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
TNIK
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 – 1:500
  • Immunohistochemistry-Paraffin 10 ug/ml

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Elephant (95%), Panda (95%), Lizard (95%), Platypus (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TNIK Antibody

  • EC 2.7.11
  • KIAA0551EC 2.7.11.1
  • TRAF2 and NCK interacting kinase
  • TRAF2 and NCK-interacting protein kinase

Background

TNIK is a MSN protein kinase that interacts with both TNF receptor-associated factor 2 (TRAF2) and the adapter protein NCK. The protein has been shown to activate the c-Jun N-terminal kinase pathway when overexpressed in Phoenix-A cells, and this activation is mediated solely by the C-terminal germinal center kinase homology region. TNIK has been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton. At least eight splice isoforms have been reported.

PMID: 24945434

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Author: premierroofingandsidinginc