TOR/mTOR Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC220457 and based on accession number NM_004958. The protein contains a C-terminal DDK tag.
|
| Specificity |
Homo sapiens FK506 binding protein 12-rapamycin associated protein 1 (FRAP1), mRNA.
|
| Gene |
MTOR
|
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
|
| Theoretical MW |
288.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
RIPA buffer
|
Lysate Details for TOR/mTOR
| Type |
Overexpression
|
| Protein State |
Native
|
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for TOR/mTOR Overexpression Lysate (Native)
- EC 2.7.11.1
- FK506 binding protein 12-rapamycin associated protein 1
- FK506 binding protein 12-rapamycin associated protein 2
- FK506-binding protein 12-rapamycin complex-associated protein 1
- FKBP12-rapamycin complex-associated protein
- FLJ44809
- FRAP
- FRAP1
- FRAP1FKBP12-rapamycin complex-associated protein 1
- FRAP2
- Mammalian target of rapamycin
- mechanistic target of rapamycin (serine/threonine kinase)
- Mechanistic target of rapamycin
- MTOR
- RAFT1
- rapamycin and FKBP12 target 1
- rapamycin associated protein FRAP2
- Rapamycin target protein 1
- RAPT1FKBP-rapamycin associated protein
- serine/threonine-protein kinase mTOR
- TOR
Background
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
