Tryptophan hydroxylase 2 [p Ser19] Antibody Summary
| Immunogen |
Synthetic phospeptide corresponding to an amino acid sequence within TPH2, which includes phosphorylated Ser19.
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| Modification |
p Ser19
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| Specificity |
NB100-66367 specifically recognizes the 55kDa Tryptophan Hydroxylase 2 (TPH2) protein, when phosphorylated at Ser19. Tryptophan Hydroxylase (TPH) is a rate-limiting enzyme which catalyses the 5-hydroxylation of tryptophan, to form the amino acid 5-hydroxytryptophan (5-HTP), in the first stage of the biosynthesis of the neurotransmitter serotonin (5-hydroxytryptamine/5HT). Mammalian TPH is encoded by two genes: TPH1 expressed in peripheral tissues e.g. skin and gut, and TPH2 primarily expressed in the brain. Studies have demonstrated the regulation/enhancement of TPH activity through phosphorylation by cAMP−dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaMKII), the latter of which phosphorylates a Ser19 site within the regulatory domain of TPH2.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
TPH2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western Blot detects a band of approximately 55kDa on recombinant TPH2 incubated in the presence of CAMKII.
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Reactivity Notes
Rat. Based on sequence similarity, is expected to react with Bovine, Zebrafish and Mouse.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
10mM HEPES (pH 7.5)
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| Preservative |
0.09% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for Tryptophan hydroxylase 2 [p Ser19] Antibody
- ADHD7
- EC 1.14.16
- EC 1.14.16.4
- FLJ37295
- MGC138872
- Neuronal tryptophan hydroxylase
- NTPH
- NTPHMGC138871
- TPH2
- tryptophan 5-hydroxylase 2
- Tryptophan 5-monooxygenase 2
- Tryptophan Hydroxylase 2
Background
TPH2 is a protein of the pterin dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH2 is expressed predominantly in the central nervous system. Mutations in TPH2 gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
