nNOS Antibody Summary
| Immunogen |
Reacts with residues 19-31 [PRKGPPLPNGDTEC] of nNOS
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| Localization |
cytoplasmic and secreted
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| Specificity |
Specific for Isoform 5 of human nNOS.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
NOS1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW |
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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Reactivity Notes
Human.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for nNOS Antibody
- bNOS
- brain
- Constitutive NOS
- EC 1.14.13.39
- IHPS1
- Neuronal NOS
- nitric oxide synthase 1 (neuronal)
- nNOS
- N-NOS
- nNOSNOS type I
- NOS
- NOS1
- Peptidyl-cysteine S-nitrosylase NOS1
Background
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including ncNOS (also designated NOS1) whose activity was first identified in neurons and maps at 12q24.2, and ecNOS (also designated NOS3), first identified in endothelial cells and mapping at 7q35-36. The inducible form of NOS, iNOS (also designated NOS2), is Ca2+-independent, expressed in a broad range of cell types and maps to 17cen -q12.
